Journal: bioRxiv

Article Title: Interferon Beta Drives Therapy Resistance in a Patient-Derived Model of High-Grade Serous Ovarian Cancer

doi: 10.64898/2026.01.27.699126

Figure Lengend Snippet: A. RT-qPCR analysis of IFN-1 RNA levels. B . Secreted IFN-1 protein levels detected in culture supernatant via HEK-Blue SEAP reporter assay. C . RT-qPCR analysis of IRDS genes. Dots indicate genes known to be transcribed by u-ISGF3. D . Intracellular flow cytometry of IFITM1, total STAT1, and pSTAT1(y701). One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*).

Article Snippet: Cells were also stained using a two-step approach first staining with IFITM1 (99969S; Cell Signaling Technology) diluted in 0.5% BSA for 1 hour at room temperature before rinsing and staining with AlexaFluorTM 647 goat anti-rabbit IgG (H+L) secondary antibody (A21245; Fisher Scientific) at room temperature for 30 minutes, washed, and resuspended in FACS stain.

Techniques: Quantitative RT-PCR, Reporter Assay, Flow Cytometry

Journal: bioRxiv

Article Title: Interferon Beta Drives Therapy Resistance in a Patient-Derived Model of High-Grade Serous Ovarian Cancer

doi: 10.64898/2026.01.27.699126

Figure Lengend Snippet: A. The IC50 of SE and CR cells was significantly higher following IFNβ treatment. B . Morphology change was determined by measuring the aspect ratio (width/legth) of 60 cells from each sample (20 cells per photo, 2 photos per well, 3 wells). Scale bar = 50µm. C . BrdU staining revealed cell cycle arrest in G0-G1 following IFNβ treatment, as measured by flow cytometry. Data represent one representative replicate. D . Proliferation was detected by counting individual wells of parental and IFNβ treated cells over time. E . RT-qPCR was used to analyze STAT1, IFITM1, and PLSCR1 following 6 passages and 12 weeks following consistent low-level IFNβ treatment. F . Intracellular flow cytometry was used to measure STAT1 and pSTAT1 protein levels following chronic IFNβ treatment. One representative replicate shown. Results are displayed as n=3, unless otherwise noted, and presented as the means ± SD. Statistical significance was determined using the unpaired t-test. p-values: p ≤ 0.05 (*) and p ≤ 0.01 (**).

Article Snippet: Cells were also stained using a two-step approach first staining with IFITM1 (99969S; Cell Signaling Technology) diluted in 0.5% BSA for 1 hour at room temperature before rinsing and staining with AlexaFluorTM 647 goat anti-rabbit IgG (H+L) secondary antibody (A21245; Fisher Scientific) at room temperature for 30 minutes, washed, and resuspended in FACS stain.

Techniques: BrdU Staining, Flow Cytometry, Quantitative RT-PCR

Journal: Burns & Trauma

Article Title: Single-cell RNA sequencing reveals the impaired epidermal differentiation and pathological microenvironment in diabetic foot ulcer

doi: 10.1093/burnst/tkae065

Figure Lengend Snippet: Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, IFITM1, S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)

Article Snippet: Dewaxed paraffin skin sections were subjected to heat-mediated antigen retrieval (citrate buffer; pH = 6) and labeled with a rabbit polyclonal antibody against IL1R2 (1: 1000; DF6682, Affinity Biosciences), a mouse monoclonal antibody against TXNIP (1: 1000; ab210826, Abcam), a rabbit monoclonal antibody against KRT15 (1: 1000; ab52816, Abcam), a rabbit monoclonal antibody against SH3KBP1 Ab (1: 1000; Ab151574, Abcam), a rabbit monoclonal antibody against GRP (1: 1000; ab236050, Abcam), a rabbit monoclonal antibody against KRT10 (1: 1000; ab76318, Abcam), a rabbit polyclonal antibody against IFITM1 (1: 100; DF2513, Affinity Biosciences), and a rabbit polyclonal antibody against IFITM3 (1: 100; DF8270, Affinity Biosciences).

Techniques: Expressing, Marker, Cell Differentiation