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Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, <t>IFITM1,</t> S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)
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Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, <t>IFITM1,</t> S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)
Rabbit Anti Ifitm2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, <t>IFITM1,</t> S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)
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Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, <t>IFITM1,</t> S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)
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Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, IFITM1, S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)

Journal: Burns & Trauma

Article Title: Single-cell RNA sequencing reveals the impaired epidermal differentiation and pathological microenvironment in diabetic foot ulcer

doi: 10.1093/burnst/tkae065

Figure Lengend Snippet: Pseudo-time analysis of KC and impaired epidermal differentiation in DFU. ( a ) Pseudo-temporal cells ordering of the total KC along the differentiation trajectory. ( b ) Pseudo-temporal cell ordering of KC in AW along the differentiation trajectory. ( c ) Pseudo-temporal cell ordering of the KC in the DFU along the differentiation trajectory. The pseudo-time data are depicted in dark purple to light yellow ( d ) The expression levels of the marker genes COL17A1, KRT14, FOS, IFITM1, S100A7, KRT6C, KRT1, and KRT10 along the pseudo-time trajectory. ( e ) UMAP analysis of the cell cycle distribution of KC at different stages. The cells are colored by type and annotated in the graph. ( f ) S score of each KC cluster. ( g ) G2M. Score of each KC cluster. * p -value <0.05. ( h ) Gene scoring analysis of the molecular signatures, including the Q score, diff score and inflammatory score. * p <0.05, ** p e <0.01, (ns) p >0.05. ( i ) Schematic of epidermal cell differentiation trajectories in AW and DFU. Quiescent score (Q score), differentiated score (diff score), basal cells (BC), diabetes-associated keratinocytes (DAK), and differentiated keratinocytes (diff-KC)

Article Snippet: Dewaxed paraffin skin sections were subjected to heat-mediated antigen retrieval (citrate buffer; pH = 6) and labeled with a rabbit polyclonal antibody against IL1R2 (1: 1000; DF6682, Affinity Biosciences), a mouse monoclonal antibody against TXNIP (1: 1000; ab210826, Abcam), a rabbit monoclonal antibody against KRT15 (1: 1000; ab52816, Abcam), a rabbit monoclonal antibody against SH3KBP1 Ab (1: 1000; Ab151574, Abcam), a rabbit monoclonal antibody against GRP (1: 1000; ab236050, Abcam), a rabbit monoclonal antibody against KRT10 (1: 1000; ab76318, Abcam), a rabbit polyclonal antibody against IFITM1 (1: 100; DF2513, Affinity Biosciences), and a rabbit polyclonal antibody against IFITM3 (1: 100; DF8270, Affinity Biosciences).

Techniques: Expressing, Marker, Cell Differentiation